By Peter Malcolm Wallis, Brian R. Hammond
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Extra info for Advances in Giardia Research
The control cysts, obtained from the feces of the five children with giardiasis who had not received the vaccine, were concentrated as described above. Results The electron microscope study of the Giardia cysts obtained from the children who had received the Shigella vaccine revealed the following: The cysts are elliptically shaped and range in length from 6 to 10 µm. Other identifiable organelles in the cytoplasm include nuclei, flagellar axonemes, rough endoplasmic reticulum, median bodies, ventral disc fragments, and ribosomes.
64:165171. 49:164171. 44:140173. 140:222228. 23:850855. 549556. p 578. Protozool. 32:383390. Gastroenterology 71:5761. 33:11791183. Microbiol. 45:6574. 80:4952. Parasitologia 4:510514. 12:851853. Science 92:416417. A. Cysts formed in vitro were both morphologically and immunologically similar to Giardia cysts formed in vivo and also were viable, as demonstrated by the uptake of fluorogenic dyes. The inability of Giardia trophozoites to undergo encystation in vitro has raised questions as to whether host related factors, such as gutassociated microorganisms or nutritive factors and stimuli from the small intestine, might play a role in this process.
The trophozoites were resuspended in TPS1 medium, so as to get counts of 1 × 104 to 1 × 107 trophozoites/mL. Cell free culture supernatant was collected from 48 h old cultures after chilling and centrifugation as described above. After washing with phosphate buffered saline, trophozoites were ruptured by a freeze/thaw cycle and then centrifuged (105 × g for 1 h) at 4°C. The supernatants (cell free extract) were used to assay the cytotoxicity. Fresh TPS1 complete medium without the parasite and minimal essential medium (MEM) with 5% of calf serum were used as controls.
Advances in Giardia Research by Peter Malcolm Wallis, Brian R. Hammond